{"data":{"id":"10.24433/co.3835580.v1","type":"dois","attributes":{"doi":"10.24433/co.3835580.v1","prefix":"10.24433","suffix":"co.3835580.v1","identifiers":[{"identifier":"1f6abf55-9614-4269-91d7-15dc51785ff4","identifierType":"Code Ocean ID"},{"identifier":"8947953","identifierType":"Code Ocean Slug"}],"alternateIdentifiers":[{"alternateIdentifierType":"Code Ocean ID","alternateIdentifier":"1f6abf55-9614-4269-91d7-15dc51785ff4"},{"alternateIdentifierType":"Code Ocean Slug","alternateIdentifier":"8947953"}],"creators":[{"name":"Barson, Daniel","nameType":"Personal","givenName":"Daniel","familyName":"Barson","affiliation":["Yale University"],"nameIdentifiers":[]},{"name":"Hamodi, Ali","nameType":"Personal","givenName":"Ali","familyName":"Hamodi","affiliation":["Yale University"],"nameIdentifiers":[]},{"name":"Xilin Shen","affiliation":["Yale University"],"nameIdentifiers":[]},{"name":"Gyorgy Lur","affiliation":["Yale University"],"nameIdentifiers":[]},{"name":"R. Todd Constable","affiliation":["Yale University"],"nameIdentifiers":[]},{"name":"Cardin, Jessica A.","nameType":"Personal","givenName":"Jessica A.","familyName":"Cardin","affiliation":["Yale University"],"nameIdentifiers":[]},{"name":"Crair, Michael C.","nameType":"Personal","givenName":"Michael C.","familyName":"Crair","affiliation":["Yale University"],"nameIdentifiers":[]}],"titles":[{"lang":"en-US","title":"Dual mesoscopic and two-photon imaging of cortical activity."}],"publisher":"Code Ocean","container":{},"publicationYear":2019,"subjects":[{"subject":"Capsule"},{"subject":"Biology"},{"subject":"imaging"},{"subject":"calcium"},{"subject":"neocortex"},{"subject":"AAV"}],"contributors":[{"name":"Higley, Michael J.","nameType":"Personal","givenName":"Michael J.","familyName":"Higley","affiliation":[],"contributorType":"ContactPerson","nameIdentifiers":[]}],"dates":[{"date":"2019-09-28","dateType":"Accepted"},{"date":"2019","dateType":"Issued"}],"language":"en","types":{"ris":"COMP","bibtex":"misc","citeproc":"article","schemaOrg":"SoftwareSourceCode","resourceType":"Capsule","resourceTypeGeneral":"Software"},"relatedIdentifiers":[],"relatedItems":[],"sizes":[],"formats":[],"version":"1.0","rightsList":[{"rights":"GNU General Public License (GPL)","rightsUri":"https://opensource.org/licenses/gpl-license"},{"rights":"Attribution-NonCommercial (CC BY-NC)","rightsUri":"https://creativecommons.org/licenses/by-nc/4.0/legalcode"}],"descriptions":[{"description":"Simultaneous mesoscopic and two-photon imaging of neuronal activity in cortical circuits Daniel Barson1,2,3,*, Ali S. Hamodi1,*, Xilin Shen4, Gyorgy Lur 1,5, R. Todd Constable2,4,6, Jessica A. Cardin1,7, Michael C. Crair1,7,8,^, Michael J. Higley1,7,9,^ Spontaneous and sensory-evoked activity propagates across varying spatial scales in the mammalian cortex, but technical challenges have limited conceptual links between the function of local neuronal circuits and brain-wide network dynamics. We present a method for simultaneous cellular-resolution two-photon calcium imaging of a local microcircuit and mesoscopic widefield calcium imaging of the entire cortical mantle in awake mice. Our multi-scale approach involves a microscope with an orthogonal axis design where the mesoscopic objective is oriented above the brain and the two-photon objective is oriented horizontally, with imaging performed through a microprism. We also introduce a viral transduction method for robust and widespread gene delivery in the mouse brain. These approaches allow us to identify the behavioral state-dependent functional connectivity of pyramidal neurons and vasoactive intestinal peptide (VIP)-expressing interneurons with long-range cortical networks. Our imaging system provides a powerful strategy for investigating cortical architecture across a wide range of spatial scales.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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